Topic: Sciences

Last updated: March 18, 2019

Scientists use a number of different methods to determine the number of microorganisms that are present in a given population. This can be accomplished by one of the method which is serial diluting the bacteria and plating the diluted bacteria on media that supports the growth of the micro-organisms. However, the latter method is another method that need more time consuming, but provides statistically accurate and repeatable results. This method is also the ideal method for enumerating microorganisms in a given population because it only identifies the living organisms in that population.
Microbial counting is useful in the basic sciences and is used determine the number of bacteria present for physiological or biochemical studies. Besides, the ability of microbial counting is to determine the safety of many foods and drugs depends on knowing the levels of microorganisms in those products.
Serial dilutions, plating and counting of live bacteria is to determine the number of bacteria in a given population. Then, we will make serial dilutions of a solution containing an unknown number of bacteria from the water sample, plate these bacteria and determine the total number of bacteria in the original solution by counting the number of colony forming units and comparing them to the dilution factor. The numbers of colony forming units (CFU’s) are divided by the product of the dilution factor and the volume of the plated diluted suspension to determine the number of bacteria per mL that were present in the original solution.


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