Peroxidase (POD, EC 1.11.1.17) activity was measured by guaiacol method according to Ballester et al. (2006). For this purpose, the reaction mixture contained 475 ?L H2O2 (100 Mm), 475 ?L guaiacol (45 Mm) and 100 ?L enzymatic extract in a total volume of 1000 ?L. The absorbance variation at 470 nm was determined for 4 min and the calculated value was assessed per 1 min.
The unit of POD activity was defined as the amount of the enzyme which decrease in absorbance 1 ?M H2O2 per min and enzyme activity was presented as unit per g of leaf fresh weight.Peroxidase (POD, EC 1.11.1.
17) activity was measured by guaiacol method according to Ballester et al. (2006). For this purpose, the reaction mixture contained 475 ?L H2O2 (100 Mm), 475 ?L guaiacol (45 Mm) and 100 ?L enzymatic extract in a total volume of 1000 ?L. The absorbance variation at 470 nm was determined for 4 min and the calculated value was assessed per 1 min.
The unit of POD activity was defined as the amount of the enzyme which decrease in absorbance 1 ?M H2O2 per min and enzyme activity was presented as unit per g of leaf fresh weight.
