Topic: Essays

Last updated: February 11, 2019

Method of DNA methylation patterns in CpG islands (or CpG-rich regions of promoter) is crutial as it allows to gather information regarding understanding various biological processes occurring in the cells, for instance, X chromosome inactivation, regulation of imprinted genes or suppressor gene silencing in tumors. Methylation-specific PCR (MSP) is conducted through modification of DNA by sodium bisulfate that converts all unmethylated but not methylated, cytosines (C) to uracil (U), and subsequent amplification with primers specific for methylated versus unmethylated DNA. Unlike previous PCR-based methods replying strictly on differential restriction enzyme cleavage in distinguishing methylated from unmethylated DNA, MSP helps to eliminate the false positive results inherent. Thus, MSP can be used as a a rapid measure in assessing the methylation status in CpG island


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