Materials and Methods
All the chemicals used in this study were procured from HiMedia, India and were of analytical grade and with high purity.
Collection of plant material
The peel of Luffa acutangula used in this study was collected from the field of Tirupattur, Vellore district, Tamil Nadu, India.
Preparation of aqueous peel extract
Take off the peel from the Luffa acutangula after washing several times with normal water and with double distilled water to remove the dust particles. Then peeled skin were kept in sun shade and dried to eliminate the residual moisture. After drying, the peel was ground into fine powder using electric mixer. The aqueous extract of Luffa acutangula was prepared by placing 10 g of dried peel powder with 1ooml of sterile double distilled water and then boiled for 60 minutes at 100 °C. This extract was cooled to room temperature and filtered using Whatman filter paper No.1. The filtered aqueous extract was stored in a refrigerator at 4 °C for further experiments.
Preparation of zinc nanoparticles
Zinc nitrate hexahydrate Zn (NO3)2.6H2O was used as precursor to synthesize ZnO nanoparticles using Luffa acutangula aqueous peel extract. For the synthesis of zinc oxide nanoparticles, 50 ml of peel aqueous extract of Luffa acutangula was taken in a clean conical flask and 5 g of Zinc nitrate was added to the solution and mixed thoroughly and kept in hot plate magnetic strirrer till water empties at 100°C.The greenish white powder formed at the bottom was scaled up and transferred to crucible. Then it was annealed at 400°C for 2 hours. The light yellow colored ZnO nano powder was thus prepared and utilized for further studies by storing in an air tight container.
Characterization of Zinc oxide nanoparticles
For analytical study UV–Vis spectra were recorded for the prepared Zinc oxide nanoparticles using UV – Vis – Varian – Cary 50 Bi in the wavelength range of 200- 600nm The FTIR spectrum was taken in the mid-IR region of 400 – 4000 cm-1 to study the functional groups. The field emission scanning electron microscopy was performed to study the morphology of the synthesized ZnO nanoparticles.


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