During doing an experiment, cleanliness is the most important thing to take care off because it is to ensure that we can get the exact result that we want from theexperiment. Thus, this technique is called aseptic techniques (Mescopio A.).
Aseptic technique is required to transfer a pure culture from one vessel to another and provided a barrier between the microorganisms in the environment with the sterile cell culture, depends upon a set of procedures to reduce the probability of contamination from these sources. The microbiologist means taking all prudent precautions to prevent contamination of the culture and spread of microorganisms into the environment by this technique. Besides, aseptic transfer technique also protects them from contamination with the culture, which should be treated as a potential pathogen. Failure on practicing aseptic technique, does not sterile the equipment and the work surface will lead to many undesired result especially in biological contamination.
This is because microorganism only can be spread and live on the normal temperature of that place. That is why we need to culture new environment that will prevent the microorganism to affect our experiment. So, aseptic technique involves avoiding any contact between the pure cultures, sterile medium, and sterile surfaces of the growth vessel with contaminating microorganisms.